Translational_Unit

Part:BBa_K622004:Design

Designed by: Fumitaka Hashiya   Group: iGEM11_Kyoto   (2011-10-01)


glnLG: coding both nitrogen regulatory protein NtrB and NtrC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 929
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1587
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We got this part directly from gDNA of E.coli by PCR.


Source

Genomic DNA of E.coli

References